Immune Profiling Nanobody Panel

The Immune Profiling Nanobody Panel is a multiplexed binder set of oligonucleotide-conjugated anti-human VHH nanobodies (~15 kDa single-domain antibodies) for simultaneous immune cell surface marker detection on the MACS Matchmaker focal molography platform.

The panel covers eight well-established leukocyte surface markers:

• CD45: pan-leukocyte (all hematopoietic cells)
• CD3ε: pan-T cell
• CD4: helper T cells
• CD8: cytotoxic T cells
• CD19: B cells
• CD56: NK cells
• CD14: monocytes
• CD7: T/NK lineage

This marker combination resolves the core immune cell classes: the T-cell compartment with helper and cytotoxic subset discrimination (CD3ε/CD4/CD8), B-cell identification (CD19), NK-cell and lymphocyte-lineage profiling (CD56/CD7), monocyte detection (CD14), and pan-leukocyte gating (CD45).

Each nanobody is conjugated to a sequence-specific oligonucleotide for DNA-directed immobilization (DDI) onto complementary capture sequences on the sensor chip surface. The nanobody format offers key advantages over conventional IgG antibodies: compact size (~15 kDa) for higher packing density on the mologram ridges, a single binding domain that eliminates Fc-mediated non-specific interactions, and a defined conjugation geometry for uniform surface orientation.

The panel runs on the Sensor [Oligo|Oligo] 8-plex for simultaneous profiling of all eight markers in a single measurement, and is compatible with complex biological matrices including whole blood, PBMCs, and serum-containing media.

Each nanobody adapter is supplied pre-conjugated to the oligonucleotide sequence of choice (Seq ID 00 to Seq ID 16), so a distinct sequence can be assigned to each binder for flexible multiplexing on the sensor chip.

Specifications

• Format: oligonucleotide-conjugated anti-human VHH nanobody, single binder per vial
• Volume: 110 µL
• Concentration: 2.6 µM
• Injections: 10 (at 200 nM, 130 µL injection volume)
• Storage solution: PBS-T

Please select the desired binder and complementary oligonucleotide sequence below for your matching sensor chip.

For research use only. Not for diagnostic or therapeutic procedures.