Polyreactivity Panel

The Polyreactivity Panel is a ready-to-use multiplexed binder set for the MACS Matchmaker focal molography platform. It quantifies non-specific binding across 8 or 16 defined liability axes in a single label-free injection on the same chip and sample as your target binding kinetics.

Two configurations are available. Essentials runs on the Sensor [Oligo|PEG] 8-plex with 8 ligands (one per major liability axis) and 8 within-chip replicates per ligand, built for the routine candidate-triage workflow at every discovery cycle. Plus runs on the Sensor [Oligo|PEG] 16-plex with 16 ligands (parallel axes per liability plus β2-glycoprotein I, LPS, IgM, fibrinogen) and 4 within-chip replicates per ligand, built for the in-depth developability assessment that supports lead-nomination and IND-enabling work.

Essentials, 8-plex composition

1. Protein A/G (Fc-capture positive control)
2. dsDNA (reference)
3. Human serum albumin (HSA)
4. Insulin
5. Lysozyme
6. Aminocardiolipin
7. Aminoheparin
8. Polyclonal human IgG

Plus, 16-plex composition

Ligands shared with Essentials are italicized; the remaining 8 are exclusive to Plus.

1. Protein A/G (Fc-capture positive control)
2. dsDNA
3. ssDNA
4. Human serum albumin (HSA)
5. Bovine serum albumin (BSA)
6. Insulin
7. Ovalbumin
8. Lysozyme
9. Aminocardiolipin
10. Aminoheparin
11. β2-glycoprotein I
12. Lipopolysaccharide (LPS)
13. Transferrin
14. Polyclonal human IgG
15. Human IgM
16. Fibrinogen

Applications

For applications, workflow, the Polyreactivity Index (PI) calculation, threshold anchoring to the Jain 2017 clinical-stage benchmark, and references, see the Polyreactivity Panel application page.

Specifications

• Format: pre-conjugated ligand mix, NHS-conjugated DDI oligos
• Volume: 110 µL
• Concentration: 2.6 µM per ligand
• Injections: 10 (at 200 nM, 130 µL injection volume)
• Storage solution: PBS-T
• Built-in QC: Protein A/G channel as per-injection Fc-capture control

For research use only. Not for diagnostic or therapeutic procedures.