Polyreactivity Panel

The Polyreactivity Panel is a ready-to-use multiplexed binder set for the MACS Matchmaker focal molography platform. It quantifies non-specific binding across 8 or 16 defined liability axes in a single label-free injection on the same chip and sample as your target binding kinetics.

Two configurations are available. Essentials runs on the Sensor [Oligo|PEG] 8-plex with 8 ligands (one per major liability axis) and 8 within-chip replicates per ligand, built for the routine candidate-triage workflow at every discovery cycle. Plus runs on the Sensor [Oligo|PEG] 16-plex with 16 ligands (parallel axes per liability plus β2-glycoprotein I, LPS, IgM, fibrinogen) and 4 within-chip replicates per ligand, built for the in-depth developability assessment that supports lead-nomination and IND-enabling work.

Essentials, 8-plex composition

1. Protein A/G (Fc-capture positive control)
2. dsDNA (reference)
3. Human serum albumin (HSA)
4. Insulin
5. Lysozyme
6. Aminocardiolipin
7. Aminoheparin
8. Polyclonal human IgG

Plus, 16-plex composition

Positions 1 to 8 are identical to the Essentials 8-plex (same ligand and same DDI oligo sequence). Positions 9 to 16 add eight further liability axes exclusive to Plus.

1. Protein A/G (Fc-capture positive control)
2. dsDNA (reference)
3. Human serum albumin (HSA)
4. Insulin
5. Lysozyme
6. Aminocardiolipin
7. Aminoheparin
8. Polyclonal human IgG
9. ssDNA
10. Bovine serum albumin (BSA)
11. Ovalbumin
12. β2-glycoprotein I
13. Lipopolysaccharide (LPS)
14. Transferrin
15. Human IgM
16. Fibrinogen

Applications

For applications, workflow, the Polyreactivity Index (PI) calculation, threshold anchoring to the Jain 2017 clinical-stage benchmark, and references, see the Polyreactivity Panel application page.

Specifications

• Format: pre-conjugated ligand mix, NHS-conjugated DDI oligos
• Volume: 110 µL
• Concentration: 2.6 µM per ligand
• Injections: 10 (at 200 nM, 130 µL injection volume)
• Storage solution: PBS-T
• Built-in QC: Protein A/G channel as per-injection Fc-capture control

For research use only. Not for diagnostic or therapeutic procedures.